GsMTx4 yog 35-amino acid peptide nrog plaub disulfide daim ntawv cog lus uas tsim cov cysteine knot motif, uas yog ib qho kev ua haujlwm zoo ntawm ntau kab laug sab venom peptides uas muaj kev ruaj ntseg thiab tshwj xeeb.Lub mechanism ntawm kev txiav txim ntawm GsMTx4 tsis yog tag nrho elucidated, tab sis nws yog ntseeg hais tias nws khi rau lub extracellular los yog transmembrane domains ntawm cationic MSCs thiab thaiv lawv pore qhib los yog gating los ntawm altering lawv conformation los yog membrane nro.GsMtx4 tau pom tias inhibit ob peb cationic MSCs nrog kev xaiv sib txawv thiab muaj zog.Piv txwv li, GsMTx4 inhibits TRPC1 nrog IC50 ntawm 0.5 μM, TRPC6 nrog IC50 ntawm 0.2 μM, Piezo1 nrog IC50 ntawm 0.8 μM, Piezo2 nrog IC50 ntawm 0.3 μM, tab sis tsis muaj kev cuam tshuam rau TRPV10 ntawm qhov siab lossis TRPV10. μM.(Bae C et al 2011, Biochemistry)
GsMtx4 tau siv los ua cov cuab yeej tshuaj los kawm txog kev ua haujlwm thiab kev tswj hwm ntawm cationic MSCs hauv ntau hom cell thiab cov ntaub so ntswg.Qee qhov piv txwv yog:
GsMtx4 tuaj yeem thaiv cov MSCs uas tau qhib los ntawm kev ncab hauv astrocytes, lub plawv hlwb, cov leeg nqaij du thiab cov leeg pob txha.Astrocytes yog lub hnub qub zoo li lub hlwb uas txhawb nqa lub hlwb thiab qaum qaum.Cov kab mob plawv yog cov hlwb uas ua rau lub plawv cov leeg.Cov leeg nqaij du yog cov hlwb uas tswj kev txav ntawm cov kabmob xws li lub plab thiab cov hlab ntsha.Cov leeg nqaij pob txha yog cov hlwb uas ua kom muaj kev yeem txav ntawm lub cev.Los ntawm thaiv cov MSCs hauv cov hlwb no, GsMtx4 tuaj yeem hloov pauv lawv cov khoom hluav taws xob, qib calcium, kev cog lus thiab so, thiab cov noob qhia.Cov kev hloov no tuaj yeem cuam tshuam li cas cov hlwb no ua haujlwm ib txwm lossis hauv cov kab mob (Suchyna li al., Xwm 2004; Bae li al., Biochemistry 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
GsMtx4 tseem tuaj yeem thaiv ib hom tshwj xeeb ntawm MSC hu ua TACAN, uas koom nrog hauv qhov mob teb.TACAN yog ib qho channel uas tau nthuav tawm hauv cov paj hlwb uas paub qhov mob.TACAN yog qhib los ntawm txhua yam stimuli, xws li siab los yog pinching, thiab ua rau mob.GsMtx4 tuaj yeem txo qhov kev ua ntawm TACAN thiab txo tus cwj pwm mob hauv cov tsiaj ua qauv ntawm kev siv tshuab (Wetzel li al., Nature Neuroscience 2007; Eijkelkamp li al., Nature Communications 2013)
GsMTx4 tuaj yeem tiv thaiv astrocytes los ntawm toxicity induced los ntawm ib tug molecule hu ua lysophosphatidylcholine (LPC), uas yog ib tug lipid mediator uas ua rau puas rau lub hlwb thiab qaum qaum.LPC tuaj yeem qhib MSCs hauv astrocytes thiab ua rau lawv noj cov calcium ntau dhau, uas ua rau oxidative stress thiab cell tuag.GsMtx4 tuaj yeem tiv thaiv LPC los ntawm kev ua kom MSCs hauv astrocytes thiab tiv thaiv lawv los ntawm toxicity.GsMtx4 tseem tuaj yeem txo cov hlwb puas thiab txhim kho kev ua haujlwm ntawm lub paj hlwb hauv cov nas uas tau txhaj LPC (Gottlieb li al., Phau ntawv Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
GsMTx4 tuaj yeem hloov kho neural qia cell sib txawv los ntawm kev thaiv ib hom MSC hu ua Piezo1, uas tau qhia hauv neural qia hlwb.Neural qia hlwb yog cov hlwb uas tuaj yeem tsim cov neurons tshiab lossis lwm hom hlwb hlwb.Piezo1 yog cov channel uas tau qhib los ntawm cov neeg kho tshuab cues los ntawm ib puag ncig, xws li nruj lossis siab, thiab cuam tshuam li cas neural qia hlwb txiav txim siab seb hom cell los ua.GsMTx4 tuaj yeem cuam tshuam nrog Piezo1 kev ua haujlwm thiab hloov pauv neural qia cell txawv ntawm cov neurons mus rau astrocytes (Pathak li al., Journal of Cell Science 2014; Lou et al., Cell Reports 2016)
Peb yog cov chaw tsim khoom polypeptide hauv Suav teb, nrog ntau xyoo ntawm kev paub tab hauv kev tsim cov polypeptide.Hangzhou Taijia Biotech Co., Ltd. yog lub chaw tsim khoom ntawm polypeptide raw khoom, uas tuaj yeem muab tau kaum tawm txhiab tus polypeptide raw cov ntaub ntawv thiab tuaj yeem kho raws li kev xav tau.Qhov zoo ntawm cov khoom polypeptide yog qhov zoo heev, thiab qhov purity tuaj yeem ncav cuag 98%, uas tau lees paub los ntawm cov neeg siv thoob plaws ntiaj teb. Zoo siab txais tos los tham nrog peb.
