Pepstatin yog pentapeptide, ib txwm tshwm sim aspartyl protease inhibitor, uas tuaj yeem cuam tshuam aspartic protease thiab acid protease ntawm ntau yam kab mob.Pepstatin feem ntau yog zais los ntawm Streptomyces hom thiab tsim los ntawm Streptomyces.Nws muaj peev xwm inhibit pepsin, pepsin D thiab angiotensin-tso enzyme, thiab muaj kev kho mob ntawm gastric ulcer, raum kub siab, mob caj dab, carrageenan edema thiab lwm yam kab mob.
Pepstatin yog ib qho muaj zog inhibitor ntawm aspartyl proteases xws li pepsin, cathepsin D, thiab renin.Qhov no ntuj pentapeptide cais los ntawm actinomycetes tau ntau xyoo cov classic renin inhibitor nyob rau hauv vitro.Pepstatin tsis yog tshwj xeeb rau renin thiab tsis zoo soluble hauv dej.Cov txheej txheem derivatives ntawm pepstatin nce nws cov solubility thiab tshwj xeeb rau renin los ntawm ntau qhov kev txiav txim ntawm qhov loj.Pepstatin muaj qhov txawv txav γ amino acid statin uas tuaj yeem hloov pauv rau ob lub amino acids ntawm daim ntawv cog lus ntawm cov protein substrate thiab thaiv substrate cleavage vim yog cov qauv kev sib piv. mus rau lub xeev hloov pauv ntawm peptide bond hydrolysis los ntawm aspartyl proteases.
Thaum cov protein raug rho tawm los ntawm cov hlwb tawg, proteases tuaj yeem raug tso tawm, uas yuav tsum tau ceev nrooj inhibited kom tsis txhob muaj protein ntau.Nyob rau hauv cov txheej txheem ntawm kev rho tawm cov protein, cov tshuaj tiv thaiv protease yuav tsum tau ntxiv los tiv thaiv proteolysis.Protease inhibitor broadly hais txog ib yam khoom uas khi rau qee pawg ntawm qhov chaw ua haujlwm ntawm protease molecules, kom cov kev ua haujlwm ntawm cov protease txo qis lossis txawm ploj mus, tab sis tsis ua rau cov enzyme protein.Qhov rhiab heev ntawm ntau yam proteases rau cov protein sib txawv yog qhov sib txawv, yog li nws yog qhov tsim nyog los kho cov concentration ntawm ntau yam proteases.Vim hais tias cov solubility ntawm protease inhibitor nyob rau hauv cov kua yog tsawg heev, nws yuav tsum tau them tshwj xeeb mloog mus rau lub protease inhibitor yuav tsum tau tag nrho tov nyob rau hauv lub buffer kom txo tau cov nag lossis daus ntawm protease inhibitor.Pepstantin A tuaj yeem inhibit acid proteases xws li pepsin, angiotensin, collagenase, cathepsin D thiab chymosin.
Pepstatin A yog ib qho inhibitor ntawm cathepsin d thiab e.Tom qab HEK293 hlwb raug kho nrog ntau qhov sib txawv ntawm pepstatin A rau 24 teev, kuaj pom qhov qhia ntawm LC3Ⅱ thiab p62.Cov txiaj ntsig tau pom tias pepstatin A tuaj yeem txhim kho qhov kev qhia ntawm LC3Ⅱ thiab P62 (P.<0.05) nyob rau hauv ib koob tshuaj.20μ g / ml pepstatin A tau siv los kho HEK293 ntawm lub sijhawm sib txawv, thiab cov teebmeem ntawm lub sijhawm sib txawv ntawm kev qhia ntawm LC3II thiab p62 tau pom.Cov txiaj ntsig tau pom tias pepstatin A tuaj yeem tswj hwm kev qhia ntawm LC3II thiab p62 nyob rau lub sijhawm.
Peb yog cov chaw tsim khoom polypeptide hauv Suav teb, nrog ntau xyoo ntawm kev paub tab hauv kev tsim cov polypeptide.Hangzhou Taijia Biotech Co., Ltd. yog lub chaw tsim khoom ntawm polypeptide raw khoom, uas tuaj yeem muab tau kaum tawm txhiab tus polypeptide raw cov ntaub ntawv thiab tuaj yeem kho raws li kev xav tau.Qhov zoo ntawm cov khoom polypeptide yog qhov zoo heev, thiab qhov purity tuaj yeem ncav cuag 98%, uas tau lees paub los ntawm cov neeg siv thoob plaws ntiaj teb. Zoo siab txais tos los tham nrog peb.
